Ctll-2 ed50

Author: grtz

August undefined, 2024

WebMeasured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. ... The ED50 for this effect is 0.05-0.25 ng/mL. The specific activity of Recombinant Human IL-2 is approximately 9.1 x 106 IU/mg, which is calibrated against recombinant human ... WebCTLL-2 cells were seeded in a 96-well plate and treated with varying concentrations of Human Recombinant IL-2. Cell proliferation was measured using a colorimetric assay. In the graph below, the specific …

IntendedUse ProductDescription

WebED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of CTLL-2 cells. Conjugate: Unconjugated : Form: Lyophilized: Concentration: 0.1 mg/mL: Purification: purified: ... Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells ... WebED50 = 0.1 - 0.4 ng/mL; determined by the dose-dependent proliferation of mouse CTLL-2 cells. Conjugate: Unconjugated : Form: Lyophilized: Purification: sequential chromatography: Contains: no preservative: ... Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of ... binding obligation crossword

LEADGMP ® IL-2 (Interleukin-2), Human - Leadgene Biomedical

WebED50 is < 10 ng/mL as determined by the dose-dependant stimulation of the proliferation of CTLL-2 cell, corresponding to a specific activity of > 1 x 10 5 IU/mg. Recommend Usage: Biological Activity ... Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. ... WebBioactivity: The ED50 of this protein, as determined by increased proliferation of mouse CTLL-2 cells stimulated with 1 ng/mL IL-2, is 0.5-1.5 µg/mL. Endotoxin: Less than 0.1 … WebIL2, IL-2, TCGF, lymphokine. Bioactivity. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. Purification Method. SDS-PAGE & … cystofix verbandswechsel

Human IL-15 Recombinant Protein (PHC9151) - Thermo Fisher Scientific

FUSION PROTEINS TARGETING TUMOUR ASSOCIATED …

WebSubculture Routine. Maintain cultures between 3-9 x 10 5 cells/ml; 5% CO 2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate … WebMeasured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.0300-0.250 ng/mL. Source. E. coli-derived human IL-2 protein Ala21-Thr153 (Cys145Ser), with and ... binding of a book crossword clueWebThe present invention relates to cancer immunotherapy. In particular, provided herein are fusion proteins for targeting tumor associated macrophages with immunostimulatory agents. binding nuts and bolts

"WebBackground: Interleukin 2 (IL-2) is a T cell stimulatory cytokine best known for inducing activated T cell proliferation . in vitro ... Bioactivity: ED50 is determined by the dose-dependent Proliferation of CTLL- 2 cells. The ED50 is typically less than 3 ng/mL. The international units of CTGrade rh IL-2. " - Ctll-2 ed50

Ctll-2 ed50

Human IL15RA protein orb594809 from Biorbyt Biocompare.com

WebJun 2, 2012 · FIGURE 61.1. A bioassay for IL-2 using CTLL-2 cells. Supernatants of tumor cells (PCl-13) transduced with the gene for human IL-2 were collected and tested for the … WebMar 25, 2024 · Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. ... The ED50 for this effect is 0.05-0.25 ng/mL. The specific activity of recombinant human IL-2 is 5.0 x 106 IU/mg, which is calibrated against the human IL-2 WHO International ...

Did you know?

WebMouse IL-2, amino acids Phe22-Gln169 (Accession# NM_008366), was expressed in E. coli . The 148 amino acid recombinant protein has a predicted molecular mass of 17.2 kD. The predicted N-terminal amino acid is Phe. >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis. Lyophilized, carrier-free. WebEach lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our IL2 CTS™ Recombinant Human growth factor is …

WebMeasure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is <0.2 ng/mL. The specific activity of recombinant human IL-2 is approximately >2.5 x 107 IU/mg. To demonstrate lot-to-lot consistency of GMP IL-2, the activity of three separate lots was examined and plotted on the same graph. Web>2 x 106 units/mg Example calculation with Gibco™ Human IL-2 Recombinant Protein (Cat. No. PHC0021) Note: If the ED 50 is expressed as a range, use the midpoint of the range. …

WebHuman IL15 (P40933, 48 a.a. - 162 a.a.) partial recombinant protein expressed in Escherichia coli. (P8346) - Products - Abnova WebCTLL2 cells growing dependent upon the use of IL2. Actually i am agrwoing ctll2 cells and they are totally grwoing well with good cell viability. and for their culture you should do …

WebED 50 =2.5 - 15 ng/ml, corresponding to a specific activity of 0.6 - 4 x 10 5 units/mg, as determined by the dose dependent stimulation of CTLL-2 cell proliferation. Application …

WebCorning ® T-75 flasks (catalog #431464) are recommended for subculturing this product. Medium Renewal: Twice per week. Some Important Considerations in Handling CTLL-2, … cystofix was ist dasWebMeasure by its ability to induce proliferation in CTLL-2 cells. The ED 50 for this effect is 3 ng/mL. ... (Interleukin-2) protein, Human (60701GMP) stimulates the proliferation of CTLL-2 cells. The ED50 is 1.5 ng/mL. IL2 (Interleukin-2) protein, Human (60701GMP) was separated on SDS-PAGE under the reducing condition and visualized by Coomassie ... binding observablecollectionWebAliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. Activity ED 50 = 0.3 - 1.8 ng/ml, corresponding to a specific activity of 0.55 - 3.3 x 10 6 units/mg, as determined by CTLL-2 cell proliferation induced by mouse IL-4 in a dose dependent manner. binding of fascia medical terminologyWebED50 : 0.0645 ng/mL; determined by dose-dependent stimulation of murine CTLL-2 cells. This corresponds to a specific activity of 1.55x10e7 IU/mg. Conjugate: Unconjugated : ... The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is 0.0645 ng/mL, corresponding to a specific activity of 1.55x107 IU/mg. Purity: >99%. binding of a fasciaWebBioactivity: ED50 is determined by the dose-dependent Proliferation of CTLL-2 cells. The ED50 is typically less than 1 ng/mL. The international units of CTGrade rh IL-15 is approximately 2.7 x 10. 5; IU/µg, which is calibrated against recombinant Human Interleukin 15 … binding offer traduccionWebIL-15 was discovered in the supernatant from a simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2 (1). Interleukin-15 (IL-15) is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts ... cyst of kidney icd-10WebEach lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our IL2 Recombinant Human growth factor is … binding offerとは