Flp-intm t-rextm 293 细胞系
WebC, immunoblots of cell lysates (input) used for FLAG immunoaffinity purification in B. D, MED21 lacking the first 5 amino acids is defective in TNFα-induced gene activation. Firefly luciferase activity was measured in lysates from Flp-InTM T-RExTM-293 cells stably expressing wild type or mutant MED21. http://www.bio-thing.com/p118025
Flp-intm t-rextm 293 细胞系
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WebFlp-In™系统使您可以稳定整合和表达感兴趣的基因,以提供单拷贝等基因细胞系。Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶标(FRT)位点。随后,包含您感兴趣的基因的表达载体会通过FRT位点的Flp重组酶介导DNA重组,整合入基因组。 WebFigure 5-9. PMA induces internalisation of hFFA4. Flp-InTM T-RExTM 293 cells able to express FLAG-hFFA4-mVenus were plated down onto poly-D-lysine-coated 30 mm glass coverslips and receptor expression was induced using doxycycline (100 ng/ml). The internalisation of hFFA4 in live cells was then imaged using a Zeiss VivaTome spinning …
Web丁香通为您提供FLPINTREX293商品详情介绍:价格:¥1 - 2000,货号:R78007,品牌:invitrogen,GIBCO,产地:美国,详见丁香通FLPINTREX293商品详情页; WebApr 2, 2024 · 基本信息. 商品名称:Epigenetics in Cancer 作者:编者:JUNJIANG FU//SABER IMANI 定价:198 出版社:科学 ISBN号:9787030543769 其他参考信息 (以实物为准). 出版时间:2024-01-01 印刷时间:2024-01-01 版次:1 印次:1 开本:16开 包装:平装 页数:364 内容提要
WebSep 29, 2024 · 293FTM 细胞 是由 Flp-InTM T-RExTM 293 ... 总之, 293系列细胞经过长时间的改造与优化,已在多个领域实现了较多的应用,源井生物对于293与293T细胞也有丰富的基因编辑和细胞模型构建的经验,并拥有上千种293细胞的KO现货,覆盖超20种信号通 … http://www.bio-thing.com/p118025
WebFeb 20, 2024 · 在我们以前的工作中,我们在四环素诱导型CMV启动子(HEK-293 Flp-In T-REx-Thermo)的控制下,建立了表达C末端3xFLAG标记的外来体成分EXOSC10(RRP6)的同基因HEK-293细胞系费雪科学)。. 该系统允许我们以与内源WT蛋白质相当的水平表达标记的EXOSC10蛋白质,并且使用磁性 ...
WebFeb 20, 2024 · 在我们以前的工作中,我们在四环素诱导型CMV启动子(HEK-293 Flp-In T-REx-Thermo)的控制下,建立了表达C末端3xFLAG标记的外来体成 … cincinnati wine festival ticketsWeb293FTM细胞则是来源于Flp-InTMT-RETM293细胞,该细胞系中转染了ecotropic receptor质粒及MAPPIT(mammalian protein-protein interaction trap)报告质粒,该细胞系主要用于 … dhx2 serviceWebFlp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression … Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes cincinnati windshieldWebThe T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal … dh x360 touchscreenWebThe Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened by hygromycin B resistance. Western blot and indirect immunofluorescence (IFA) were used to examine the expression of the target protein in ... dhx40 and heartWebFigure 3-20. Relative expression of FLAG-hFFA4-mVenus and FLAG-hFFA4-GFP2 in a Flp-InTM T-RExTM 293 cell line. Flp-InTM T-RExTM 293 double stable cells able to express inducibly FLAG-hFFA4-mVenus and constitutively FLAG-hFFA4-GFP2 were seeded into poly-Dlysine coated black 96-well plates. Cells were treated with varying concentrations … cincinnati wineries on the riverWebMar 26, 2024 · Protocol 2 Protein complex purification from Flp-InTM T-RExTM 293 stably expressed FLAG tagged protein cell line Protocol 3 ChiP-Sequencing from Flp-InTM T-RExTM 293 stably expressed FLAG tagged protein cell lines Protocol 4 ChiP assay for endogenous gene Protocol 5 The cancer cell index, migration, and invasion assays Color … dhx9 molecular weight