WebThe Escherichia coli signal recognition particle (SRP) receptor, FtsY, plays a fundamental role in co-translational targeting of membrane proteins via the SRP pathway. Efficient targeting relies on membrane interaction of FtsY and heterodimerization with the SRP protein Ffh, which is driven by detachment of α helix (αN1) in FtsY. WebNov 16, 2004 · Besides the NG domain, Ffh has a C-terminal methionine-rich domain, called the M domain ().The M domain provides the binding module for 4.5S RNA (18, 19), which facilitates Ffh·FtsY complex formation (20, 21).It is widely accepted that the M domain also contains the primary binding site for signal sequences (7, 22), although recent data …
The Structural Basis of FtsY Recruitment and GTPase Activation by …
WebApr 8, 2014 · His-tagged variants of Ffh, FtsY, and SecA were over-expressed in E. coli and purified from cell extracts by metal affinity chromatography. The SecYEG complex was … http://walterlab.ucsf.edu/wp-content/uploads/2015/04/Peluso_Biochemistry2001.pdf dashun foundation
Protein Interactomes of Streptococcus mutans YidC1 and YidC2 …
WebMar 8, 2024 · The SRP system has a core consisting of the proteins, FtsY and Ffh, which derive from a single FtsY/Ffh ancestral protein. The system is also associated with a … Web× GTPase activity × Erkennt Signalpeptid im GTP gebundenen Zustand × Ribonukleoprotein besteht aus RNA und Proteine × A-Domain: interaction with YecYEG and membrane × M-Domain: methionine rich, high affinity RNA binding and recognition of signal sequence × Helical N-Domain: interaction with ribosome × G-Domain: GTPase × ffH protein: N,G ... WebJan 15, 2004 · The FtsY–Ffh complex forms a composite active site at the interface. In this site, many catalytic residues interact both with the substrate in cis and with residues across the interface in trans ... dashu hair products