Web14175095: 500 mL: 价格 413.00. 您的 ... Gibco™ HBSS 在位于纽约格兰德岛、符合 cGMP 质量管理要求的工厂生产。该工厂是在 FDA 注册的医疗器械生产商,通过 ISO 13485 标准认证。为确保供应链的稳定,我们同时提 … WebWash tissue with PBS for 3 times. Mince tissue into small pieces until no more than 1mm. Add 1mL 2% collagenase I, II, IV to the tissue. Incubate tissue at 37°C for 10 minutes, then pipette gently to disperse the large pieces of your tissue. Repeat 5th step for 3 …
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WebJun 18, 2024 · HBSS: Gibco: Cat#14175095: FCS: Cell Biologics: Cat#6912: Pen/Strep/L-Glut: Merck: Cat#G6784: Corn oil: Sigma-Aldrich: Cat#C8267-500ML: Experimental models: organisms/strains; ... Place the lung pieces in 15 mL Falcon tubes and incubate in 5 mL of 0.5% collagenase type IV in HBSS for 45 min at 37°C while rotating. b. Web14175095. 14175095. Hanks' Balanced Salt Solution (HBSS) (1X), liquid, contains no calcium chloride, magnesium chloride, magnesium sulfate, or phenol red, 500mL (Gibco) View Detail Add to Order. 109 $13.36 14025092. 14025092. Web(Composition of wash buffer: HBSS supplemented with 1% Penicillin/Streptomycin, 0.25 mg/mL Amphotericin B /10 mg/mL Gentamicin, 1% Kanamycin, 5% FCS). 10.3 Vortex and Incubate in 37oC shaker for 15-45 mins 10.4 Check for small clusters of cells under a microscope every 15 min. 10.5 Add 5ml pancreatic wash buffer to tissue. (1:1 v/v) feit wifi smart bulbs costco