Smad3 molecular weight
WebbSMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in … WebbThis antibody detects endogenous protein at a molecular weight of 55 and 60 kDa. Purity is >95% by SDS-PAGE. Target Information Smad proteins, the mammalian homologs of the …
Smad3 molecular weight
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WebbCalculated molecular weight: 48 kDa: Observed molecular weight: 48 kDa: GenBank accession number: NM_005902: Gene symbol: SMAD3: Gene ID (NCBI) 4088: Conjugate: … WebbMass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Keywords: (E)-SIS3 521984-48-5 TGF-beta/Smad Transforming growth factor beta Smad3 …
WebbSimple Western™ analysis of lysates (1 mg/mL) from serum-starved HT1080 cells treated with hTGF-beta3 (10 ng/mL, 30 min) using Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520. The virtual lane view (left) shows the target band (as indicated) at a 1:250 dilution of primary antibody. WebbConstitutive proteasomal degradation of steady-state Smad3 is regulated by Axin. ( A) HaCaT cells were pretreated with 10 μM SB-431542 for 6 h, and 50 μg/mL CHX or 25 μM MG-132 was then added as indicated. Total cell lysates were probed for endogenous Smad3 and Smad2. γ-Tubulin was used as a loading control.
Webb12 dec. 2024 · Human_Smad3 GGCT complex: 0.02 M sodium potassium phosphate, 0.1 M BisTris propane pH 6.5, 20% PEG3350 cryoprotected with 20% 550 MME. Crystals were … WebbIP. IF. Confocal immunofluorescent analysis of HT1080 cells, serum-starved (left), or treated with hTGF-β3 #8425 (100 ng/ml, 30 min; right), …
Webb28 mars 2024 · SMADs consist of eight known proteins, which can be divided into three sub-groups as follows; SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8 (also known as SMAD9) are receptor-regulated SMADs (R-SMADs), which function as substrates for TGF-B receptors, and therefore play a role in the signaling pathway of TGF-B superfamily of …
WebbWhen using primary antibodies produced in mouse to detect proteins with a molecular weight in the range of 50 kDa, we recommend using Rabbit Anti-Mouse IgG (Light Chain … how much are glastonbury hospitality ticketsSmads are roughly between 400 and 500 amino acids long, and consist of two globular regions at the amino and carboxy termini, connected by a linker region. These globular regions are highly conserved in R-Smads and Co-Smads, and are called Mad homology 1 (MH1) at the N-terminus, and MH2 at the C-terminus. The MH2 domain is also conserved in I-Smads. The MH1 domain is primarily involved in DNA binding, while the MH2 is responsible for the interaction with other Sm… how much are glow plugsWebbWe measured the expression levels of proteins involved in the wrinkle formation pathway, c-Jun N-terminal kinase (JNK)/c-FOS/c-Jun/MMPs/Smad3 pathway, and mRNA expression levels of elasticity factors—transforming growth factor-β receptor I (TGF-β RI), procollagen type I, and collagen type I—in the dorsal skin of mice exposed to UVB … how much are glofish at petsmartWebb1 apr. 2024 · Phospho-SMAD3 (Ser423, Ser425) Polyclonal Antibody Invitrogen catalog: 44-246G total number of products: 41 domestic rabbit polyclonal reactivity: human, mouse modification: phosphorylated application: western blot, immunocytochemistry citations: 5 quantity: 100 µL price: US 482 to the supplier SMAD3 phospho S423/phospho S425 … how much are glue in hair extensionsWebbThe molarity calculator equation Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Keywords: SIS3 1009104-85-1 SIS 3 SIS-3 TGF-beta/Smad Transforming growth factor beta Smad3 phosphorylation myofibroblast differentiation fibroblasts TGF-β1 Inhibitor inhibitor inhibit Your Recently Viewed Products: how much are glucose test stripsWebbFind smad3 and related products for scientific research at MilliporeSigma. US EN. Applications Products Services Support. Advanced Search. Structure Search. Search … photography trade show boothWebb7 apr. 2024 · To further explore the necessary role of Smad3 in SARS-CoV-2 N-induced AKI, we overexpressed the SARS-CoV-2 N protein in the kidneys of Smad3 KO-db/m, Smad3 WT-db/m, Smad3 KO-db/db, and... photography toddlers